Dual Wavelength Infrared CO₂ Control System

The SANYO single beam, dual detector infrared CO₂ system offers unprecedented control accuracy and stability by simultaneously measuring two wavelengths.

The chamber sample and a reference is configured around a ceramic-based sensor linked to the microprocessor controller with a sophisticated P.I.D./R™ (proportional, integral and derivative) algorithm. Benefits include ultra-fast recovery without overshoot and accurate CO₂ averages during periods of frequent incubator access with multiple door openings.

• The ceramic-based technique is impervious to changes in temperature and relative humidity, highly stable during door openings, and sensitive to restoration of setpoint equilibrium when the door is closed.
• The sensor maintains a continuous auto-zero function for accuracy.
• Actual CO₂ is displayed on the main control panel.
• A CO₂ sample port mounted on the incubator front permits convenient confirmation of chamber CO₂ density.
• An optional automatic CO₂ switchover system is available; see Accessories. A two-stage regulator from the supply cylinder to the incubator is required; see Accessories.

SafeCell UV System

Sterisonic GxP Models –UVH and –UV include SafeCell™ UV contamination control technology based on an integrated combination of narrow bandwidth, ozone-free ultraviolet light, ceramic infrared CO₂ control, InCu SaFe™ copper-enriched stainless steel alloy interiors and Direct Heat and Air Jacket™ heating managed by a microprocessor controller.

These incubators offer the industry's most stable cell culture environment and are useful for the most critical applications where continuous contamination control is essential to cell viability. SANYO SafeCell™ UV Series CO₂ incubators offer significant economic benefits by minimizing interruptions for decontamination, by improving cell culture growth and expression under stable, repeatable conditions, and by minimizing the potential for product loss.

• SafeCell™ UV includes a programmable ultraviolet lamp, isolated from cell cultures, that sterilizes conditioned air and humidity reservoir water to prevent contamination without affecting cell cultures in vitro.
• SafeCell™ UV inhibits the growth of mycoplasma, bacteria, molds, spores, yeasts and fungi without costly HEPA filter air scrubbers which accumulate contaminants in the chamber air.
• High temperature decontamination systems, which can actually encourage in vitro growth of heat resistant thermophilic and hyperthermophilic microorganisms, are avoided.
• InCu SaFe™ interior surfaces provide natural resistance to contamination.
• The SafeCell™ UV lamp reduces H₂O₂ to trace sterile water condensate following the seven-minute vapor sterilization process.

  Active Background Contamination Control™

A continuous Active Background Contamination Control™ process eliminates contamination without downtime.

At the base of the plenum an isolated beam of high intensity, ozone-free ultraviolet light destroys contaminants in the air and in the humidity water reservoir, away from active cell cultures.

• Contaminants trapped within the distilled water pan are destroyed by ultraviolet light.
• Sterile, humidified air is released from the lower plenum for vertical convection through and around the perforated shelves. Interior air motion is suspended when the door is opened, minimizing movement of room air contaminants into the chamber.
• UV light is isolated by the plenum cover to protect cell cultures
• Airborne contaminants are eliminated by an automatic UV cycle that automatically turns ON for a specified period after each door opening.
• Trace contaminants that attach to interior surfaces are destroyed by the passive germicidal properties of the InCu SaFe™ surfaces.

H₂O₂ Sterilization

The unique Sterisonic™ GxP H₂O₂ sterilization system limits downtime to less than three hours when total chamber sterilization is desired.

• All interior components and CO₂ sampling loop are sterilized in situ; no need for removal and autoclaving.
• Available as standard on the MCO-19AIC(UVH); optional on the MCO-19AIC(UV).
• Following sterilization, SANYO Active Background Contamination Control™ fights contamination while cell culture protocols are in process.

InCu SaFe™ Construction for Germicidal Protection

SANYO offers exclusive use of InCu SaFe™ copper-enriched stainless steel alloy interior surfaces within a technical design created to eliminate contamination sources and to mitigate the effect of airborne contaminates introduced through normal use.

• Selected to provide natural germicidal protection without rust or corrosion, InCu SaFe™ expresses a natural germicidal attribute to inhibit the growth of molds, fungi, mycoplasma and bacteria.
• All interior components, including the air management plenum, shelf supports, humidity pan and blower wheel assembly are easily removable without tools if required.
• During the H₂O₂ sterilization cycle interior components can be repositioned within the chamber for in situ sterilization.
• When components are removed, all interior surfaces are exposed for conventional wipe down.
• Large curve corners and electropolished surfaces are easy to clean.
• Pass thru ports accommodate probes or instrumentation leads as required for specialized cell culture protocols.
• Each chamber includes a port positioned in the interior, rear wall, upper left, with dual, non-VOC silicone stoppers inside and outside the cabinet for added protection.

Mycoplasma Survival Results

Chart summarizes test results with four strains of mycoplasma. Results demonstrate how SANYO InCu SaFe™ copper enriched stainless steel alloy offers germicidal properties of conventional C1100 copper while maintaining both corrosion-proof and discoloration-resistant properties of conventional Type 304 stainless steel. Detailed test results are available from SANYO.

  Direct Heat and Air Jacket™ Heating System

The patented Direct Heat and Air Jacket™ surrounds the inner walls with a natural convection airflow that converts to radiant wall heat through thermal conduction to achieve accurate, uniform and highly responsive temperature control within the chamber.

• The microprocessor controller directs proportional distribution of power to independent heating sources surrounding the chamber.
• Arranged in three zones, these sources include the side, top and rear walls, the chamber base and the outer door.
• Each zone is controlled by the microprocessor which manages continuous feedback from the incubator chamber sensors.

Zone		Location	Function	Energy	Microprocessor Controller
	Main	Side, top and rear walls	Dominant heat source	Variable	Energizes any, all or a combination of heating elements as required.
	Base	Floor	Base heater elevates the humidity reservoir to achieve 95%RH at 37°C	Variable
	Front	Outer door	Warms the inner glass in response to ambient conditions; eliminates condensation on glass and around the opening and promotes temperature uniformity	Variable
	Air Jacket	Side, top and rear walls	Sealed, surrounds interior chamber with natural air convection	--	--
	Insulation	Side, top and rear walls, door	Promotes energy efficiency, mitigates effect of ambient temperature fluctuations on air jacket	--	--